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Image Search Results
Journal: The Plant journal : for cell and molecular biology
Article Title: Convergent evolution of hetero-oligomeric cellulose synthesis complexes in mosses and seed plants
doi: 10.1111/tpj.14366
Figure Lengend Snippet: Summary of results from membrane-based yeast two-hybrid (MbYTH)/bimolecular fluorescence complementation (BiFC) protein interaction assays. Weak interaction (W) for MbYTH were more than twice the negative controls, but less than half of the positive control.
Article Snippet: For
Techniques: Membrane, Fluorescence, Control
Journal: PLoS ONE
Article Title: Hypothalamic-Specific Manipulation of Fto , the Ortholog of the Human Obesity Gene FTO , Affects Food Intake in Rats
doi: 10.1371/journal.pone.0008771
Figure Lengend Snippet: ( A ) Fto expression, quantified by real-time RT-PCR, is up-regulated in the arcuate nucleus following a high-fat diet demonstrated by the relative arcuate Fto mRNA expression in rat either fed on a standard chow or on a high-fat diet for 10 weeks. Data is represented as the mean±S.E.M of at least 6 independent rats per group; **P<0.01. ( B ) Fto mRNA is diffusely expressed throughout the arcuate nucleus and overlaps with Pomc neurons. Double in situ hybridization detecting Fto ( 35 S labeled) and Pomc (DIG labeled) mRNA in the cells of the arcuate nucleus. Insert in figure shows a high-magnification (scale bar, 20 µm) of a Pomc containing neurons colocalised with Fto (marked with *). ( C ) Intra-nuclei bilateral injections of adeno-associated virus (AAV2/7) mediated transfer of GFP cDNA precisely targets the hypothalamic arcuate (ARC) and paraventricular (PVN) nuclei; as demonstrated by photomicrographs of representative coronal sections showing localization of GFP 7 days after injection; right panels, higher magnification of area indicated by red box. Scale bar, 100µm. 3v: third ventricle; ME: median eminence.
Article Snippet: Dig-labelled probes for sense and antisense Pomc mRNA were generated from the cDNA template corresponding to exon3 of
Techniques: Expressing, Quantitative RT-PCR, In Situ Hybridization, Labeling, Injection